Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27040 | 5' | -55.8 | NC_005832.1 | + | 23619 | 0.66 | 0.830175 |
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Target: 5'- gUCggGCagGGCGGCGUaaGGCAGGCcgGCGu -3' miRNA: 3'- -AGa-CGa-CCGCUGUAagCUGUCCG--CGU- -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 87519 | 0.66 | 0.821426 |
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Target: 5'- --gGCUGGCGGcCAUguaccaCGACGGGCu-- -3' miRNA: 3'- agaCGACCGCU-GUAa-----GCUGUCCGcgu -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 103576 | 0.67 | 0.784682 |
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Target: 5'- aUCUcGCgcaGGCGACcgUCGA-GGGCgGCAg -3' miRNA: 3'- -AGA-CGa--CCGCUGuaAGCUgUCCG-CGU- -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 23503 | 0.67 | 0.755556 |
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Target: 5'- -aUGCUGGUgGACAUgUGGCuGGCGUu -3' miRNA: 3'- agACGACCG-CUGUAaGCUGuCCGCGu -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 33719 | 0.7 | 0.63131 |
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Target: 5'- -aUGCUGGCGGCcaaaGGCAGG-GCGu -3' miRNA: 3'- agACGACCGCUGuaagCUGUCCgCGU- -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 54753 | 0.72 | 0.466423 |
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Target: 5'- cCUGUgccUGGCGGCGgacgUCGACAGGUacuuugaGCAg -3' miRNA: 3'- aGACG---ACCGCUGUa---AGCUGUCCG-------CGU- -5' |
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| 27040 | 5' | -55.8 | NC_005832.1 | + | 15730 | 1.09 | 0.001894 |
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Target: 5'- gUCUGCUGGCGACAUUCGACAGGCGCAc -3' miRNA: 3'- -AGACGACCGCUGUAAGCUGUCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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