Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27041 | 5' | -47.5 | NC_005832.1 | + | 87120 | 0.66 | 0.998229 |
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Target: 5'- cCAGCcUCAuggucAGGGCCUUCuUUGACg -3' miRNA: 3'- -GUCGaAGUuc---UCUCGGAAGcAAUUGa -5' |
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| 27041 | 5' | -47.5 | NC_005832.1 | + | 85534 | 0.67 | 0.996326 |
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Target: 5'- gAGCUUgGAGAGcgcGUCUUCGUcaaUGACc -3' miRNA: 3'- gUCGAAgUUCUCu--CGGAAGCA---AUUGa -5' |
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| 27041 | 5' | -47.5 | NC_005832.1 | + | 85685 | 0.68 | 0.987585 |
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Target: 5'- aGGCUaCAGGAGAGCaggUCaUUGACg -3' miRNA: 3'- gUCGAaGUUCUCUCGga-AGcAAUUGa -5' |
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| 27041 | 5' | -47.5 | NC_005832.1 | + | 83442 | 0.68 | 0.987585 |
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Target: 5'- uCGGCcagaGAGAGGGCCggCGggGACa -3' miRNA: 3'- -GUCGaag-UUCUCUCGGaaGCaaUUGa -5' |
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| 27041 | 5' | -47.5 | NC_005832.1 | + | 104910 | 0.7 | 0.960512 |
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Target: 5'- aGGCUgagCAAGAGGGCCUcCGUc---- -3' miRNA: 3'- gUCGAa--GUUCUCUCGGAaGCAauuga -5' |
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| 27041 | 5' | -47.5 | NC_005832.1 | + | 15922 | 1.09 | 0.011517 |
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Target: 5'- gCAGCUUCAAGAGAGCCUUCGUUAACUg -3' miRNA: 3'- -GUCGAAGUUCUCUCGGAAGCAAUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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