Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27047 | 3' | -46.6 | NC_005832.1 | + | 34199 | 0.66 | 0.998571 |
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Target: 5'- -aUUAcgUUCAUgGCAGUCUCCAaACg -3' miRNA: 3'- uaAAUa-AGGUAgUGUCAGAGGUcUGa -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 69475 | 0.67 | 0.998255 |
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Target: 5'- -----cUCCAUCcaAGUCUCCAG-CUg -3' miRNA: 3'- uaaauaAGGUAGugUCAGAGGUCuGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 69553 | 0.67 | 0.998255 |
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Target: 5'- -----cUCCAUCcaAGUCUCCAG-CUg -3' miRNA: 3'- uaaauaAGGUAGugUCAGAGGUCuGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 69631 | 0.67 | 0.998255 |
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Target: 5'- -----cUCCAUCcaAGUCUCCAG-CUg -3' miRNA: 3'- uaaauaAGGUAGugUCAGAGGUCuGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 5874 | 0.68 | 0.995665 |
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Target: 5'- ------cUCGUCACGGUCaugCCGGGCUc -3' miRNA: 3'- uaaauaaGGUAGUGUCAGa--GGUCUGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 37695 | 0.68 | 0.995591 |
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Target: 5'- --gUAUUCUAUCGCaagauguAGUCUuuGGGCUa -3' miRNA: 3'- uaaAUAAGGUAGUG-------UCAGAggUCUGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 99698 | 0.7 | 0.979177 |
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Target: 5'- ---aAUUCUgacaCACAGUCUCCAuGACUg -3' miRNA: 3'- uaaaUAAGGua--GUGUCAGAGGU-CUGA- -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 8808 | 0.81 | 0.539298 |
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Target: 5'- --aUAUUUUAUCGCaAGUCUCCAGACg -3' miRNA: 3'- uaaAUAAGGUAGUG-UCAGAGGUCUGa -5' |
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| 27047 | 3' | -46.6 | NC_005832.1 | + | 21560 | 1.08 | 0.017105 |
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Target: 5'- gAUUUAUUCCAUCACAGUCUCCAGACUa -3' miRNA: 3'- -UAAAUAAGGUAGUGUCAGAGGUCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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