Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27051 | 5' | -58.9 | NC_005832.1 | + | 87363 | 0.66 | 0.706616 |
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Target: 5'- uCAGGCUCUCCa-GGgUGAGGGuGg-- -3' miRNA: 3'- cGUCCGAGAGGgaCCaGCUCCC-Caau -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 27873 | 0.66 | 0.655349 |
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Target: 5'- gGCAGGCccuggacCUCCUUaGGcgCGAGGGGc-- -3' miRNA: 3'- -CGUCCGa------GAGGGA-CCa-GCUCCCCaau -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 65449 | 0.67 | 0.623252 |
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Target: 5'- gGCAGGCgUCUCUCcgaaccgUGGgcgaGAGGGGg-- -3' miRNA: 3'- -CGUCCG-AGAGGG-------ACCag--CUCCCCaau -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 48740 | 0.68 | 0.572741 |
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Target: 5'- uCGGGCauuguuauaUCUCUCUGuGUCGAgaaaccGGGGUUAa -3' miRNA: 3'- cGUCCG---------AGAGGGAC-CAGCU------CCCCAAU- -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 94917 | 0.68 | 0.562533 |
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Target: 5'- cGCAGGCccCUgCCCcGGcggUGAGGGGUa- -3' miRNA: 3'- -CGUCCGa-GA-GGGaCCa--GCUCCCCAau -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 9865 | 0.68 | 0.562532 |
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Target: 5'- gGCAGGCUggagcugucugCUCCCUacgguucuucGGagGAGGGGa-- -3' miRNA: 3'- -CGUCCGA-----------GAGGGA----------CCagCUCCCCaau -5' |
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| 27051 | 5' | -58.9 | NC_005832.1 | + | 27660 | 1.08 | 0.000987 |
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Target: 5'- aGCAGGCUCUCCCUGGUCGAGGGGUUAa -3' miRNA: 3'- -CGUCCGAGAGGGACCAGCUCCCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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