Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27053 | 3' | -43.6 | NC_005832.1 | + | 103034 | 0.66 | 0.99999 |
Target: 5'- aGAGAgcauAUCCUgAGAGAua-UCUUCUGu -3' miRNA: 3'- -CUCUau--UAGGA-UCUCUuucAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 69013 | 0.66 | 0.999979 |
Target: 5'- uGGAUcccuAUCCcaauggccgGGGGAAAGUCUUCUu -3' miRNA: 3'- cUCUAu---UAGGa--------UCUCUUUCAGAAGAu -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 75450 | 0.67 | 0.999945 |
Target: 5'- gGAGAgGAUCCUAGAGAcuAGGUUc---- -3' miRNA: 3'- -CUCUaUUAGGAUCUCU--UUCAGaagau -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 58065 | 0.68 | 0.999867 |
Target: 5'- aGAGu--AUCCUcGAGGAGGauUCUUCUAc -3' miRNA: 3'- -CUCuauUAGGAuCUCUUUC--AGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 28597 | 0.69 | 0.999701 |
Target: 5'- ------cUCUUucAGAGAAAGUCUUCUAa -3' miRNA: 3'- cucuauuAGGA--UCUCUUUCAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 73453 | 0.69 | 0.999701 |
Target: 5'- aGAGAUAccucacucuuAUCCUuaaaucuucugAGAGAAAGUCUUg-- -3' miRNA: 3'- -CUCUAU----------UAGGA-----------UCUCUUUCAGAAgau -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 51367 | 0.71 | 0.996745 |
Target: 5'- ------cUUUUAGAGAAAGUCUUCUGa -3' miRNA: 3'- cucuauuAGGAUCUCUUUCAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 30543 | 0.74 | 0.977485 |
Target: 5'- gGAGAUAAUCUUGuGAGAGAuacUCUUCUGa -3' miRNA: 3'- -CUCUAUUAGGAU-CUCUUUc--AGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 98152 | 0.75 | 0.96824 |
Target: 5'- cGGGAUAGUCUUccGGAGAGUCUUCc- -3' miRNA: 3'- -CUCUAUUAGGAucUCUUUCAGAAGau -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 106280 | 0.79 | 0.848798 |
Target: 5'- cGAGAgucuUCUgAGAGAAAGUCUUCUGa -3' miRNA: 3'- -CUCUauu-AGGaUCUCUUUCAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 30787 | 0.79 | 0.848798 |
Target: 5'- uGAGAUAcUUUgugAGAGAAAGUCUUCUGa -3' miRNA: 3'- -CUCUAUuAGGa--UCUCUUUCAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 30459 | 0.99 | 0.116425 |
Target: 5'- gGAGAaaUAAUCCUAGAGAAAGUCUUCUGa -3' miRNA: 3'- -CUCU--AUUAGGAUCUCUUUCAGAAGAU- -5' |
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27053 | 3' | -43.6 | NC_005832.1 | + | 30400 | 1.09 | 0.030229 |
Target: 5'- aGAGAUAAUCCUAGAGAAAGUCUUCUAa -3' miRNA: 3'- -CUCUAUUAGGAUCUCUUUCAGAAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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