Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27053 | 5' | -49.5 | NC_005832.1 | + | 83414 | 0.66 | 0.986155 |
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Target: 5'- ----uAGAAGAgGGC-CUGGAGCUc- -3' miRNA: 3'- gaaucUCUUCUgCCGaGAUCUCGAuu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 59906 | 0.66 | 0.986155 |
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Target: 5'- -cUGGAuGAGGA-GGCUCUGGAGg--- -3' miRNA: 3'- gaAUCU-CUUCUgCCGAGAUCUCgauu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 10119 | 0.67 | 0.977394 |
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Target: 5'- --cAGAGuuuGACGGCUCcagGGAGUg-- -3' miRNA: 3'- gaaUCUCuu-CUGCCGAGa--UCUCGauu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 19458 | 0.67 | 0.968207 |
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Target: 5'- ---uGAGAAGGCGGC-CUGGgagucuuGGCUGc -3' miRNA: 3'- gaauCUCUUCUGCCGaGAUC-------UCGAUu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 102246 | 0.68 | 0.961414 |
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Target: 5'- --aAGAGGAGACaGaUCUGGAGCg-- -3' miRNA: 3'- gaaUCUCUUCUGcCgAGAUCUCGauu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 82727 | 0.68 | 0.953241 |
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Target: 5'- --cAGAGAuucAGACGGCUC-AGAGaCUc- -3' miRNA: 3'- gaaUCUCU---UCUGCCGAGaUCUC-GAuu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 63932 | 0.71 | 0.847793 |
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Target: 5'- --cAGAGAGGGCGGCagaguacgCUGGAGUa-- -3' miRNA: 3'- gaaUCUCUUCUGCCGa-------GAUCUCGauu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 17751 | 0.73 | 0.781609 |
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Target: 5'- --cGGAGggGGCuggGGCUCUGGGGUUu- -3' miRNA: 3'- gaaUCUCuuCUG---CCGAGAUCUCGAuu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 92543 | 0.74 | 0.729315 |
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Target: 5'- ---cGAcGggGACGGCUCUGG-GCUGc -3' miRNA: 3'- gaauCU-CuuCUGCCGAGAUCuCGAUu -5' |
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| 27053 | 5' | -49.5 | NC_005832.1 | + | 30365 | 0.92 | 0.081097 |
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Target: 5'- uCUUAGGaAAGACGGCUCUAGAGCUAAu -3' miRNA: 3'- -GAAUCUcUUCUGCCGAGAUCUCGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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