Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27055 | 5' | -59.7 | NC_005832.1 | + | 87732 | 0.66 | 0.61505 |
Target: 5'- uUGGGaGUCucUGGGGUgcucaUGGGCUCCa--- -3' miRNA: 3'- cACCC-CAG--ACCCUA-----ACCCGAGGgagu -5' |
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27055 | 5' | -59.7 | NC_005832.1 | + | 72843 | 0.67 | 0.604752 |
Target: 5'- --aGGGUCUGGGGccUGaGCgCCCUCAg -3' miRNA: 3'- cacCCCAGACCCUa-ACcCGaGGGAGU- -5' |
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27055 | 5' | -59.7 | NC_005832.1 | + | 54014 | 0.67 | 0.574014 |
Target: 5'- gGUGaGGUCUGGGAgagacGGaGC-CCCUCu -3' miRNA: 3'- -CACcCCAGACCCUaa---CC-CGaGGGAGu -5' |
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27055 | 5' | -59.7 | NC_005832.1 | + | 20343 | 0.67 | 0.557763 |
Target: 5'- -aGGGGUcCUGGGAgauggacaguccgGGGC-CCUUCu -3' miRNA: 3'- caCCCCA-GACCCUaa-----------CCCGaGGGAGu -5' |
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27055 | 5' | -59.7 | NC_005832.1 | + | 25358 | 0.7 | 0.402579 |
Target: 5'- aGUGuGGGaaaGGGucUGGGCUCCCUCu -3' miRNA: 3'- -CAC-CCCagaCCCuaACCCGAGGGAGu -5' |
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27055 | 5' | -59.7 | NC_005832.1 | + | 32186 | 1.08 | 0.000937 |
Target: 5'- uGUGGGGUCUGGGAUUGGGCUCCCUCAg -3' miRNA: 3'- -CACCCCAGACCCUAACCCGAGGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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