miRNA: 3'- gGCCGGGA--CCGCAUGCGGCGGAGgca -5'
miRNA: 3'- -GGCCGGGACCGCAuGCggCg-GAGGca -5'
miRNA: 3'- -GGCCGGG--ACC-GCAUgCGGCGGaGGCa -5'
miRNA: 3'- -GGCCGGGACCGCAUGCGGCGGAGGCA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27058 | 5' | -63.9 | NC_005832.1 | + | 77365 | 0.7 | 0.267427 |
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Target: 5'- aUGGUCUUucGGUGUACGCCGCCUUg-- -3' miRNA: 3'- gGCCGGGA--CCGCAUGCGGCGGAGgca -5' |
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| 27058 | 5' | -63.9 | NC_005832.1 | + | 40288 | 0.71 | 0.25543 |
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Target: 5'- gUCGGCCCUGGUGU-CGuuGgaCUCCa- -3' miRNA: 3'- -GGCCGGGACCGCAuGCggCg-GAGGca -5' |
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| 27058 | 5' | -63.9 | NC_005832.1 | + | 76188 | 0.72 | 0.222076 |
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Target: 5'- cCUGGUCCugUGGaCGUAgGCCGCC-CCGa -3' miRNA: 3'- -GGCCGGG--ACC-GCAUgCGGCGGaGGCa -5' |
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| 27058 | 5' | -63.9 | NC_005832.1 | + | 36648 | 1.08 | 0.000461 |
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Target: 5'- cCCGGCCCUGGCGUACGCCGCCUCCGUg -3' miRNA: 3'- -GGCCGGGACCGCAUGCGGCGGAGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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