Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27079 | 5' | -60 | NC_005832.1 | + | 32441 | 0.67 | 0.622089 |
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Target: 5'- --cGUGGa--CAGGUCCCaCGgGGaCCCu -3' miRNA: 3'- cuuCACCcaaGUCCAGGG-GCgCC-GGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 72649 | 0.66 | 0.642434 |
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Target: 5'- ---aUGGGcccgCGGGg-CCCGaCGGCCCa -3' miRNA: 3'- cuucACCCaa--GUCCagGGGC-GCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 43790 | 0.66 | 0.652599 |
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Target: 5'- aGAGGcUGGGUUUcgacgagauGG-CCCaCGgGGCCCu -3' miRNA: 3'- -CUUC-ACCCAAGu--------CCaGGG-GCgCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 104140 | 0.66 | 0.67893 |
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Target: 5'- cAGGUGGGcaCAGGccauguugucuacCCCCacggcguccgcgGCGGCCCu -3' miRNA: 3'- cUUCACCCaaGUCCa------------GGGG------------CGCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 19943 | 0.66 | 0.682962 |
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Target: 5'- -uGG-GGGUggAGGUCCUCGgGGCa- -3' miRNA: 3'- cuUCaCCCAagUCCAGGGGCgCCGgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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