Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27079 | 5' | -60 | NC_005832.1 | + | 79689 | 0.67 | 0.571485 |
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Target: 5'- ---uUGGGgagCAGGUuggCCCUGaGGCCCg -3' miRNA: 3'- cuucACCCaa-GUCCA---GGGGCgCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 80832 | 0.71 | 0.385783 |
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Target: 5'- cAGG-GGGUcgCGGuGUcCCCCGgGGCCCu -3' miRNA: 3'- cUUCaCCCAa-GUC-CA-GGGGCgCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 94121 | 0.71 | 0.394121 |
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Target: 5'- uAGG-GGGUUCugcuuggccacGGUCUCUGCGGCCUu -3' miRNA: 3'- cUUCaCCCAAGu----------CCAGGGGCGCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 104140 | 0.66 | 0.67893 |
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Target: 5'- cAGGUGGGcaCAGGccauguugucuacCCCCacggcguccgcgGCGGCCCu -3' miRNA: 3'- cUUCACCCaaGUCCa------------GGGG------------CGCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 104857 | 0.68 | 0.565467 |
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Target: 5'- ----aGGGUUCAGGUagagugucccaaaacCCCCGUGGaguggaacacCCCu -3' miRNA: 3'- cuucaCCCAAGUCCA---------------GGGGCGCC----------GGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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