miRNA: 3'- cuucACCCa----AGUCCa-----GGGgCGCCGGG- -5'
miRNA: 3'- cuUCaCCCAagUCCAGGGGCgCCGgg -5'
miRNA: 3'- -CUUCaCCCAag--UCCAGGG-GCGCCGgg -5'
miRNA: 3'- cuucacCCA-AGUCCAGGGGC-GCCG--GG- -5'
miRNA: 3'- -CUUCAC---CCAa--GUCCa-GGGGCGCCGGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27079 | 5' | -60 | NC_005832.1 | + | 26455 | 0.69 | 0.474121 |
|
Target: 5'- ---cUGGGgagugUCGGGcagccaCCCgGCGGCCCu -3' miRNA: 3'- cuucACCCa----AGUCCa-----GGGgCGCCGGG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 19943 | 0.66 | 0.682962 |
|
Target: 5'- -uGG-GGGUggAGGUCCUCGgGGCa- -3' miRNA: 3'- cuUCaCCCAagUCCAGGGGCgCCGgg -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 7267 | 0.68 | 0.561464 |
|
Target: 5'- gGGAGaGGGUggcaAGGUCCUaCGCGGCg- -3' miRNA: 3'- -CUUCaCCCAag--UCCAGGG-GCGCCGgg -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 7097 | 0.67 | 0.601774 |
|
Target: 5'- -----aGGUcUCAGGUCCCCGgCGGUgaCCa -3' miRNA: 3'- cuucacCCA-AGUCCAGGGGC-GCCG--GG- -5' |
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| 27079 | 5' | -60 | NC_005832.1 | + | 5766 | 0.72 | 0.330766 |
|
Target: 5'- cGAGGUGucuGGUggaCGGGgaUCCCGUGGCCCu -3' miRNA: 3'- -CUUCAC---CCAa--GUCCa-GGGGCGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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