Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27084 | 5' | -56.3 | NC_005832.1 | + | 97582 | 0.66 | 0.779726 |
Target: 5'- --aUgGCGGCCGCGGcauCCcAGCUCa -3' miRNA: 3'- gaaAgUGUCGGCGCCaccGGuUCGAG- -5' |
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27084 | 5' | -56.3 | NC_005832.1 | + | 9904 | 0.66 | 0.820452 |
Target: 5'- ---cCGCGGCCgacgcgcccgacgaaGCGGgcaggGGaCCGGGCUCc -3' miRNA: 3'- gaaaGUGUCGG---------------CGCCa----CC-GGUUCGAG- -5' |
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27084 | 5' | -56.3 | NC_005832.1 | + | 70737 | 0.66 | 0.816885 |
Target: 5'- ---cCGCAGCCGCGGcuauaGCCAgAGC-Ca -3' miRNA: 3'- gaaaGUGUCGGCGCCac---CGGU-UCGaG- -5' |
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27084 | 5' | -56.3 | NC_005832.1 | + | 5203 | 0.66 | 0.807842 |
Target: 5'- ---gCACGacgaccccGCCGUcgagggucuGGUGGCCcAGCUCa -3' miRNA: 3'- gaaaGUGU--------CGGCG---------CCACCGGuUCGAG- -5' |
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27084 | 5' | -56.3 | NC_005832.1 | + | 61934 | 0.66 | 0.789253 |
Target: 5'- --gUUugGGuuGUGGUGGCCGucgggguuAGuCUCg -3' miRNA: 3'- gaaAGugUCggCGCCACCGGU--------UC-GAG- -5' |
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27084 | 5' | -56.3 | NC_005832.1 | + | 95686 | 0.66 | 0.816885 |
Target: 5'- ----aGCGGCCGCGGaGGCU-AGCcCg -3' miRNA: 3'- gaaagUGUCGGCGCCaCCGGuUCGaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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