Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27084 | 5' | -56.3 | NC_005832.1 | + | 95938 | 0.66 | 0.807842 |
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Target: 5'- ----aGCGGCCGCGGccagGGCagCAAGCcCg -3' miRNA: 3'- gaaagUGUCGGCGCCa---CCG--GUUCGaG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 47712 | 0.66 | 0.807842 |
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Target: 5'- --aUgGCGGCCGCGaGgccGGCCAAGg-- -3' miRNA: 3'- gaaAgUGUCGGCGC-Ca--CCGGUUCgag -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 70737 | 0.66 | 0.816885 |
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Target: 5'- ---cCGCAGCCGCGGcuauaGCCAgAGC-Ca -3' miRNA: 3'- gaaaGUGUCGGCGCCac---CGGU-UCGaG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 4690 | 0.66 | 0.816885 |
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Target: 5'- aCUUcCACGaCCGaGGUGGCCAGGauCUCc -3' miRNA: 3'- -GAAaGUGUcGGCgCCACCGGUUC--GAG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 95686 | 0.66 | 0.816885 |
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Target: 5'- ----aGCGGCCGCGGaGGCU-AGCcCg -3' miRNA: 3'- gaaagUGUCGGCGCCaCCGGuUCGaG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 9904 | 0.66 | 0.820452 |
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Target: 5'- ---cCGCGGCCgacgcgcccgacgaaGCGGgcaggGGaCCGGGCUCc -3' miRNA: 3'- gaaaGUGUCGG---------------CGCCa----CC-GGUUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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