Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27084 | 5' | -56.3 | NC_005832.1 | + | 94020 | 0.66 | 0.798628 |
|
Target: 5'- --aUgGCGGUCGCGGUGGCgAuuuGGUa- -3' miRNA: 3'- gaaAgUGUCGGCGCCACCGgU---UCGag -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 95686 | 0.66 | 0.816885 |
|
Target: 5'- ----aGCGGCCGCGGaGGCU-AGCcCg -3' miRNA: 3'- gaaagUGUCGGCGCCaCCGGuUCGaG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 95938 | 0.66 | 0.807842 |
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Target: 5'- ----aGCGGCCGCGGccagGGCagCAAGCcCg -3' miRNA: 3'- gaaagUGUCGGCGCCa---CCG--GUUCGaG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 97119 | 0.71 | 0.531313 |
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Target: 5'- ----gGCcGCCGCGGUGGCCcugagggcaaAGGCUg -3' miRNA: 3'- gaaagUGuCGGCGCCACCGG----------UUCGAg -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 97245 | 0.66 | 0.779726 |
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Target: 5'- --cUCAgGGCCaccGCGGcGGCCu-GCUCc -3' miRNA: 3'- gaaAGUgUCGG---CGCCaCCGGuuCGAG- -5' |
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| 27084 | 5' | -56.3 | NC_005832.1 | + | 97582 | 0.66 | 0.779726 |
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Target: 5'- --aUgGCGGCCGCGGcauCCcAGCUCa -3' miRNA: 3'- gaaAgUGUCGGCGCCaccGGuUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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