Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27094 | 3' | -52.3 | NC_005832.1 | + | 66466 | 0.67 | 0.94596 |
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Target: 5'- aCGgGCAUGG-GCGaagACGgcUGCGGGa -3' miRNA: 3'- gGCgUGUACCuCGUg--UGCaaACGCUC- -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 9400 | 0.68 | 0.913756 |
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Target: 5'- cCCGgGCGUGGAGCcACACGguccagccCGAa -3' miRNA: 3'- -GGCgUGUACCUCG-UGUGCaaac----GCUc -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 82036 | 0.69 | 0.887109 |
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Target: 5'- gCCGUgGCAaaGGGGCACACGUaUGCGu- -3' miRNA: 3'- -GGCG-UGUa-CCUCGUGUGCAaACGCuc -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 46976 | 0.69 | 0.845814 |
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Target: 5'- aCGCGgGUGGAguaGCACACGUUcuuagcuaucaugaUGCuGGGg -3' miRNA: 3'- gGCGUgUACCU---CGUGUGCAA--------------ACG-CUC- -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 6113 | 0.7 | 0.804193 |
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Target: 5'- cCUGCcuCAUGGAGguuCGCGgcUGCGAGg -3' miRNA: 3'- -GGCGu-GUACCUCgu-GUGCaaACGCUC- -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 23503 | 0.76 | 0.483989 |
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Target: 5'- aUGCugGUGGAcaugugGCugGCGUUUGCGAu -3' miRNA: 3'- gGCGugUACCU------CGugUGCAAACGCUc -5' |
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| 27094 | 3' | -52.3 | NC_005832.1 | + | 68768 | 1.12 | 0.002948 |
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Target: 5'- gCCGCACAUGGAGCACACGUUUGCGAGg -3' miRNA: 3'- -GGCGUGUACCUCGUGUGCAAACGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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