miRNA: 3'- cuauaUUUguaGGA-GUGAGGGUGGUG- -5'
miRNA: 3'- cuauaUUUGuAGGAGUGa---GGGugGUG- -5'
miRNA: 3'- cuauaUUUGUAGGAGUgAGG---GUGGUG- -5'
miRNA: 3'- cuauaUUUGUAGGaGugAGGGUGGug -5'
miRNA: 3'- -CUAUAUUUGUAGGaGUGAGGGuGGUg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27096 | 5' | -47.9 | NC_005832.1 | + | 66274 | 0.67 | 0.994752 |
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Target: 5'- ----gGGAag-CCUcCAUUCCCGCCGCg -3' miRNA: 3'- cuauaUUUguaGGA-GUGAGGGUGGUG- -5' |
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| 27096 | 5' | -47.9 | NC_005832.1 | + | 90282 | 0.67 | 0.994752 |
|
Target: 5'- ----cGAGCcUCCUCGCguggUCCugCACg -3' miRNA: 3'- cuauaUUUGuAGGAGUGa---GGGugGUG- -5' |
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| 27096 | 5' | -47.9 | NC_005832.1 | + | 56694 | 0.66 | 0.996825 |
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Target: 5'- ----cAAACAUCCUCAgUUCgugCGCCAUg -3' miRNA: 3'- cuauaUUUGUAGGAGUgAGG---GUGGUG- -5' |
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| 27096 | 5' | -47.9 | NC_005832.1 | + | 54797 | 0.66 | 0.997342 |
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Target: 5'- ----cAGGCGUCC-CugUCCCACUc- -3' miRNA: 3'- cuauaUUUGUAGGaGugAGGGUGGug -5' |
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| 27096 | 5' | -47.9 | NC_005832.1 | + | 25151 | 0.66 | 0.997788 |
|
Target: 5'- aGGUGUAAACAUCUaUAUUCCC-UCAg -3' miRNA: 3'- -CUAUAUUUGUAGGaGUGAGGGuGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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