Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 271 | 3' | -48.2 | AC_000008.1 | + | 9543 | 0.66 | 0.939522 |
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Target: 5'- gCCGCCCGUCau--GUCCCgGUUAUggGUu -3' miRNA: 3'- -GGUGGGUAGauauCAGGG-UAGUAa-CA- -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 16900 | 0.66 | 0.927863 |
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Target: 5'- -gACCgGUCUGUGGUCaCGUCGUg-- -3' miRNA: 3'- ggUGGgUAGAUAUCAGgGUAGUAaca -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 5891 | 0.67 | 0.900652 |
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Target: 5'- -aGCUCAgCUAUAGUCCUGUCccUGUa -3' miRNA: 3'- ggUGGGUaGAUAUCAGGGUAGuaACA- -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 8290 | 0.68 | 0.876921 |
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Target: 5'- gCCGCCgAUCUGaaauGUCCCGUCc---- -3' miRNA: 3'- -GGUGGgUAGAUau--CAGGGUAGuaaca -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 29893 | 0.69 | 0.831557 |
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Target: 5'- aCGCUCAUCUGcAGcCUCAUCAcUGUg -3' miRNA: 3'- gGUGGGUAGAUaUCaGGGUAGUaACA- -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 6194 | 0.75 | 0.500937 |
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Target: 5'- uCCGUCCaAUCUAUGGaUUCCAUCAUUGUu -3' miRNA: 3'- -GGUGGG-UAGAUAUC-AGGGUAGUAACA- -5' |
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| 271 | 3' | -48.2 | AC_000008.1 | + | 29644 | 1.12 | 0.002152 |
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Target: 5'- aCCACCCAUCUAUAGUCCCAUCAUUGUg -3' miRNA: 3'- -GGUGGGUAGAUAUCAGGGUAGUAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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