Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27101 | 5' | -61.5 | NC_005832.1 | + | 44170 | 0.7 | 0.350778 |
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Target: 5'- uACCggcgggCACGGUCCUGGUGGg--GaAGGUg -3' miRNA: 3'- -UGGa-----GUGCCGGGACCGCCacaC-UCCG- -5' |
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| 27101 | 5' | -61.5 | NC_005832.1 | + | 60223 | 0.7 | 0.350778 |
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Target: 5'- uGCC-CAUGGCCagcuUGGCGuaggcuagGUGAGGCa -3' miRNA: 3'- -UGGaGUGCCGGg---ACCGCca------CACUCCG- -5' |
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| 27101 | 5' | -61.5 | NC_005832.1 | + | 90921 | 0.7 | 0.343124 |
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Target: 5'- aGCCUCAucuCGGCCgagGGCGGgccccucagGAGGCu -3' miRNA: 3'- -UGGAGU---GCCGGga-CCGCCaca------CUCCG- -5' |
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| 27101 | 5' | -61.5 | NC_005832.1 | + | 80324 | 0.71 | 0.320893 |
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Target: 5'- uCCUCACGGgucCCCUGGUccagucGGaUG-GAGGCg -3' miRNA: 3'- uGGAGUGCC---GGGACCG------CC-ACaCUCCG- -5' |
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| 27101 | 5' | -61.5 | NC_005832.1 | + | 53991 | 0.77 | 0.130529 |
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Target: 5'- gACCuUCAUGGCCCUGGagcuGUGgagGAGGCu -3' miRNA: 3'- -UGG-AGUGCCGGGACCgc--CACa--CUCCG- -5' |
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| 27101 | 5' | -61.5 | NC_005832.1 | + | 75875 | 1.11 | 0.000422 |
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Target: 5'- gACCUCACGGCCCUGGCGGUGUGAGGCg -3' miRNA: 3'- -UGGAGUGCCGGGACCGCCACACUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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