Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27102 | 3' | -59.2 | NC_005832.1 | + | 76095 | 1.05 | 0.001365 |
Target: 5'- cAAGAGGUGAGCGUUGCGCACCCCGCAc -3' miRNA: 3'- -UUCUCCACUCGCAACGCGUGGGGCGU- -5' |
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27102 | 3' | -59.2 | NC_005832.1 | + | 30159 | 0.67 | 0.553507 |
Target: 5'- cAAGAGGcuuGCGgUGCGCucCCCUGCGa -3' miRNA: 3'- -UUCUCCacuCGCaACGCGu-GGGGCGU- -5' |
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27102 | 3' | -59.2 | NC_005832.1 | + | 7311 | 0.67 | 0.605116 |
Target: 5'- gGAGAGGcugUGGGUGUccUGUGCGCCCaGUc -3' miRNA: 3'- -UUCUCC---ACUCGCA--ACGCGUGGGgCGu -5' |
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27102 | 3' | -59.2 | NC_005832.1 | + | 6685 | 0.66 | 0.657234 |
Target: 5'- -cGGGuGUGAGUGcgGCGCACgucaCCGCc -3' miRNA: 3'- uuCUC-CACUCGCaaCGCGUGg---GGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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