Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27111 | 3' | -54.8 | NC_005832.1 | + | 99602 | 0.66 | 0.863245 |
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Target: 5'- gAGCUGC-CCgaAGCGGgagAG-GCUCCc -3' miRNA: 3'- gUCGACGuGG--UCGUCaa-UCaCGAGGu -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 5348 | 0.67 | 0.779967 |
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Target: 5'- gAGCUGgGCCaccagacccucgacGGCGGggucgucGUGCUCCAg -3' miRNA: 3'- gUCGACgUGG--------------UCGUCaau----CACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 45857 | 0.68 | 0.711629 |
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Target: 5'- gCAGacgGCACCAgGCuagAGUacgUGGUGCUCCGu -3' miRNA: 3'- -GUCga-CGUGGU-CG---UCA---AUCACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 16526 | 0.72 | 0.529854 |
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Target: 5'- gGGCUGCACCAGCGagUAGUaCUCg- -3' miRNA: 3'- gUCGACGUGGUCGUcaAUCAcGAGgu -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82576 | 0.73 | 0.439759 |
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Target: 5'- cCAGCUGCACCAGCAcc-AGcUGCaCCAg -3' miRNA: 3'- -GUCGACGUGGUCGUcaaUC-ACGaGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82415 | 0.9 | 0.0381 |
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Target: 5'- ----aGCACCAGCAGUUAGUGCUCCAg -3' miRNA: 3'- gucgaCGUGGUCGUCAAUCACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82441 | 1.09 | 0.00194 |
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Target: 5'- cCAGCUGCACCAGCAGUUAGUGCUCCAg -3' miRNA: 3'- -GUCGACGUGGUCGUCAAUCACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82486 | 1.09 | 0.00194 |
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Target: 5'- cCAGCUGCACCAGCAGUUAGUGCUCCAg -3' miRNA: 3'- -GUCGACGUGGUCGUCAAUCACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82531 | 1.09 | 0.00194 |
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Target: 5'- cCAGCUGCACCAGCAGUUAGUGCUCCAg -3' miRNA: 3'- -GUCGACGUGGUCGUCAAUCACGAGGU- -5' |
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| 27111 | 3' | -54.8 | NC_005832.1 | + | 82606 | 1.09 | 0.00194 |
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Target: 5'- cCAGCUGCACCAGCAGUUAGUGCUCCAg -3' miRNA: 3'- -GUCGACGUGGUCGUCAAUCACGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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