Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27112 | 3' | -55.8 | NC_005832.1 | + | 95343 | 0.67 | 0.77806 |
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Target: 5'- ---aGCCGGGGCGGUCUgGGu-UCUGc -3' miRNA: 3'- caacCGGCUCCGUCAGAgUCucAGAC- -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 45459 | 0.68 | 0.718117 |
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Target: 5'- ----uUCGGGGCAGgccCUCAGAGUCUu -3' miRNA: 3'- caaccGGCUCCGUCa--GAGUCUCAGAc -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 97334 | 0.68 | 0.718117 |
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Target: 5'- --cGGCCGAGGCcugCUCcaGGAGcCUGu -3' miRNA: 3'- caaCCGGCUCCGucaGAG--UCUCaGAC- -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 62312 | 0.68 | 0.68694 |
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Target: 5'- cUUGGCguaaGAGGCAGagugcUUUCcGAGUCUGg -3' miRNA: 3'- cAACCGg---CUCCGUC-----AGAGuCUCAGAC- -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 64115 | 0.7 | 0.591791 |
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Target: 5'- --gGGCCGAGGCuGUCgUgAGAGUUc- -3' miRNA: 3'- caaCCGGCUCCGuCAG-AgUCUCAGac -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 85643 | 0.7 | 0.570778 |
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Target: 5'- -aUGGCaggugcgGGGGCuucAGcCUCAGAGUCUGa -3' miRNA: 3'- caACCGg------CUCCG---UCaGAGUCUCAGAC- -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 35178 | 0.71 | 0.559297 |
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Target: 5'- -gUGGCCucuguggGAGaCAGcCUCAGGGUCUGg -3' miRNA: 3'- caACCGG-------CUCcGUCaGAGUCUCAGAC- -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 10250 | 0.73 | 0.431599 |
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Target: 5'- -aUGGCCuGGGCGGUCUCGGuGUa-- -3' miRNA: 3'- caACCGGcUCCGUCAGAGUCuCAgac -5' |
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| 27112 | 3' | -55.8 | NC_005832.1 | + | 84657 | 1.09 | 0.001769 |
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Target: 5'- cGUUGGCCGAGGCAGUCUCAGAGUCUGa -3' miRNA: 3'- -CAACCGGCUCCGUCAGAGUCUCAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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