Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27112 | 5' | -54.1 | NC_005832.1 | + | 16385 | 0.67 | 0.878518 |
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Target: 5'- aGCGGACAggGUGAcguuaaGGCUGGCguUAGu -3' miRNA: 3'- cCGUCUGUaaCACU------CCGGCCGacAUC- -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 10797 | 0.67 | 0.855198 |
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Target: 5'- -cCGGACAggagGUGGGGaUCGGCUGcGGa -3' miRNA: 3'- ccGUCUGUaa--CACUCC-GGCCGACaUC- -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 33175 | 0.67 | 0.846977 |
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Target: 5'- cGGCGGACAcgGUGGgugcGGCCGacacGgUGUGGu -3' miRNA: 3'- -CCGUCUGUaaCACU----CCGGC----CgACAUC- -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 64256 | 0.68 | 0.829908 |
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Target: 5'- uGGCuGugAcUGUGAuGGCCGGCg---- -3' miRNA: 3'- -CCGuCugUaACACU-CCGGCCGacauc -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 80357 | 0.68 | 0.829908 |
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Target: 5'- uGGUGGACAggGUGGGGaaGGCcaggGUGu -3' miRNA: 3'- -CCGUCUGUaaCACUCCggCCGa---CAUc -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 12985 | 0.7 | 0.734353 |
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Target: 5'- cGCAGACGcccccgacGAGGCCGaGCUGUGc -3' miRNA: 3'- cCGUCUGUaaca----CUCCGGC-CGACAUc -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 98123 | 0.71 | 0.639818 |
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Target: 5'- cGUGGACAUUggaGUGAGGaCGGCcGUAGg -3' miRNA: 3'- cCGUCUGUAA---CACUCCgGCCGaCAUC- -5' |
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| 27112 | 5' | -54.1 | NC_005832.1 | + | 84621 | 1.11 | 0.002122 |
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Target: 5'- cGGCAGACAUUGUGAGGCCGGCUGUAGc -3' miRNA: 3'- -CCGUCUGUAACACUCCGGCCGACAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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