miRNA: 3'- gCCUCAACGuuuCUUG-GACCaCCACc -5'
miRNA: 3'- gCCUCAACguuucUUGGACCACCacc -5'
miRNA: 3'- -GCCUCAa-CGuuUCuuGGACCACCACc -5'
miRNA: 3'- gCCUCaacguuUCUUGGACCACCacc -5'
miRNA: 3'- gcCUCA-ACGu---UUCUUgGACCACCaCC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27121 | 5' | -51.2 | NC_005832.1 | + | 20410 | 0.67 | 0.960316 |
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Target: 5'- uGGGGUUGC---GGACgCUGGgGGUGa -3' miRNA: 3'- gCCUCAACGuuuCUUG-GACCaCCACc -5' |
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| 27121 | 5' | -51.2 | NC_005832.1 | + | 76216 | 0.67 | 0.963948 |
|
Target: 5'- aGGGGUUGac---GGCCUGGUGGg-- -3' miRNA: 3'- gCCUCAACguuucUUGGACCACCacc -5' |
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| 27121 | 5' | -51.2 | NC_005832.1 | + | 37029 | 0.67 | 0.967341 |
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Target: 5'- uCGGGGUcgGUguAGuuUCUGGUGGUGu -3' miRNA: 3'- -GCCUCAa-CGuuUCuuGGACCACCACc -5' |
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| 27121 | 5' | -51.2 | NC_005832.1 | + | 75378 | 0.66 | 0.980968 |
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Target: 5'- gGGAGaaaacuGGGACCUGGUGGa-- -3' miRNA: 3'- gCCUCaacguuUCUUGGACCACCacc -5' |
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| 27121 | 5' | -51.2 | NC_005832.1 | + | 101726 | 0.66 | 0.983084 |
|
Target: 5'- -aGAGUcUGCcguuGAGAAgCUGGUGG-GGu -3' miRNA: 3'- gcCUCA-ACGu---UUCUUgGACCACCaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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