Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27124 | 5' | -43.7 | NC_005832.1 | + | 40645 | 0.66 | 0.999998 |
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Target: 5'- uGUCUGAgGcAGAAUAAu--CUGAUGc -3' miRNA: 3'- gCAGACUgC-UCUUAUUuguGACUAUc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 67788 | 0.66 | 0.999998 |
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Target: 5'- aCGUCagGGcCGAGGAgcguCAUUGAUAGc -3' miRNA: 3'- -GCAGa-CU-GCUCUUauuuGUGACUAUC- -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 92494 | 0.66 | 0.999998 |
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Target: 5'- -cUCUGACGAGGAgAGGCAgaGGc-- -3' miRNA: 3'- gcAGACUGCUCUUaUUUGUgaCUauc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 90955 | 0.68 | 0.999942 |
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Target: 5'- aUGUCguUGACGGGAGUGGACuuuacCUGGa-- -3' miRNA: 3'- -GCAG--ACUGCUCUUAUUUGu----GACUauc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 103545 | 0.68 | 0.999942 |
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Target: 5'- aGUCUggGugGAGggUGGACACg----- -3' miRNA: 3'- gCAGA--CugCUCuuAUUUGUGacuauc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 92823 | 0.69 | 0.999697 |
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Target: 5'- -cUCUGugGAGAu--GAgGCUGAUGa -3' miRNA: 3'- gcAGACugCUCUuauUUgUGACUAUc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 36954 | 0.7 | 0.999612 |
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Target: 5'- aGUCUGACGAGGAccuugaaGGAgGCggcGGUAGg -3' miRNA: 3'- gCAGACUGCUCUUa------UUUgUGa--CUAUC- -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 62093 | 0.7 | 0.999507 |
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Target: 5'- uGUCccgagGACGGGGAUGGACACgGGg-- -3' miRNA: 3'- gCAGa----CUGCUCUUAUUUGUGaCUauc -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 92974 | 0.77 | 0.940853 |
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Target: 5'- uCGUCaGACGAuggaGAAUAAACuCUGAUGGg -3' miRNA: 3'- -GCAGaCUGCU----CUUAUUUGuGACUAUC- -5' |
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| 27124 | 5' | -43.7 | NC_005832.1 | + | 93090 | 1.12 | 0.026034 |
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Target: 5'- uCGUCUGACGAGAAUAAACACUGAUAGa -3' miRNA: 3'- -GCAGACUGCUCUUAUUUGUGACUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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