Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2713 | 3' | -57.9 | NC_001491.2 | + | 122212 | 0.66 | 0.813911 |
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Target: 5'- cAGCGCUagcgcggguuguaGGGUGGUGguuguUGGUGGggGCa -3' miRNA: 3'- uUCGCGG-------------UCCACCAC-----GCCACUuaCGc -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 15762 | 0.66 | 0.797028 |
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Target: 5'- -cGCGCgAGGUcugGGUGCuuGUGGcuGUGCGg -3' miRNA: 3'- uuCGCGgUCCA---CCACGc-CACU--UACGC- -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 97221 | 0.67 | 0.720731 |
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Target: 5'- cGAGCGCCugaagaugcccuGGGUGGUGCGGauc-UGUc -3' miRNA: 3'- -UUCGCGG------------UCCACCACGCCacuuACGc -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 17385 | 0.68 | 0.690602 |
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Target: 5'- gGGGCGCCAGagcGUGCGGgauaGAUGCGc -3' miRNA: 3'- -UUCGCGGUCcacCACGCCac--UUACGC- -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 28536 | 0.68 | 0.690602 |
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Target: 5'- uGGCuaCAGGcaaaGGUGC-GUGAAUGCGg -3' miRNA: 3'- uUCGcgGUCCa---CCACGcCACUUACGC- -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 13928 | 0.68 | 0.670256 |
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Target: 5'- -cGCGCCGGGgcuggcggccuUGG-GCGGUGGcaGCGc -3' miRNA: 3'- uuCGCGGUCC-----------ACCaCGCCACUuaCGC- -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 125744 | 0.71 | 0.524956 |
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Target: 5'- aGAGCGgCGGGUGGagaagacaccguagUG-GGUGAGUGUGg -3' miRNA: 3'- -UUCGCgGUCCACC--------------ACgCCACUUACGC- -5' |
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| 2713 | 3' | -57.9 | NC_001491.2 | + | 131313 | 1.07 | 0.002258 |
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Target: 5'- aAAGCGCCAGGUGGUGCGGUGAAUGCGa -3' miRNA: 3'- -UUCGCGGUCCACCACGCCACUUACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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