Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2713 | 5' | -56.8 | NC_001491.2 | + | 31794 | 0.66 | 0.85252 |
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Target: 5'- cGUGUAuGUAUGCCGCcuauauuCGCCUgGGGg -3' miRNA: 3'- aCGCGU-CAUAUGGUGu------GUGGGgCCCg -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 148964 | 0.66 | 0.855635 |
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Target: 5'- aGCGCGGcggagggccuggGCCACGCcggGgCUCGGGCc -3' miRNA: 3'- aCGCGUCaua---------UGGUGUG---UgGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 106285 | 0.66 | 0.860248 |
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Target: 5'- gGCGCAacucUGUGCCucauuacgaccCACACgCCgGGGCg -3' miRNA: 3'- aCGCGUc---AUAUGGu----------GUGUG-GGgCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 137615 | 0.66 | 0.860248 |
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Target: 5'- cGcCGCGGca-GCgGC-CGCCCCGGGg -3' miRNA: 3'- aC-GCGUCauaUGgUGuGUGGGGCCCg -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 135299 | 0.66 | 0.860248 |
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Target: 5'- cGCuGCAGUuu-CCAUACA-CCCGGuGUa -3' miRNA: 3'- aCG-CGUCAuauGGUGUGUgGGGCC-CG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 23282 | 0.66 | 0.867028 |
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Target: 5'- gGCGC--UAUACCuucgcaaGCGCGCaaCGGGCg -3' miRNA: 3'- aCGCGucAUAUGG-------UGUGUGggGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 124449 | 0.66 | 0.867771 |
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Target: 5'- cGCGCAcGUGUcugcagaaaACCccgaagACAC-CCCCGuGGCc -3' miRNA: 3'- aCGCGU-CAUA---------UGG------UGUGuGGGGC-CCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 140131 | 0.66 | 0.889058 |
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Target: 5'- cGCGCcGUGUcggcggagGCagcggACGCGCUCCGGaGCg -3' miRNA: 3'- aCGCGuCAUA--------UGg----UGUGUGGGGCC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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