Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2713 | 5' | -56.8 | NC_001491.2 | + | 112955 | 0.66 | 0.880075 |
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Target: 5'- gGCGCGGgcccgugacccuugACCAgAUAUggCCCGGGUa -3' miRNA: 3'- aCGCGUCaua-----------UGGUgUGUG--GGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 148583 | 0.66 | 0.882181 |
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Target: 5'- cGCGCGGUGgcuCUGCgcgGCGCCcuccucaaaguCCGGGUc -3' miRNA: 3'- aCGCGUCAUau-GGUG---UGUGG-----------GGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 137737 | 0.66 | 0.889058 |
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Target: 5'- gGCgGCGGgcgcCCGCGgGuCCCCGGGg -3' miRNA: 3'- aCG-CGUCauauGGUGUgU-GGGGCCCg -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 121876 | 0.66 | 0.889058 |
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Target: 5'- cGCGCAGcggGaaGCuCugCUCGGGCa -3' miRNA: 3'- aCGCGUCauaUggUGuGugGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 121044 | 0.66 | 0.889058 |
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Target: 5'- cUGCGU-GUGUACC-CACACCUggugauccgcaGGGUa -3' miRNA: 3'- -ACGCGuCAUAUGGuGUGUGGGg----------CCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 75016 | 0.66 | 0.889058 |
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Target: 5'- gGCGgAGUuUGCUGCaaACGCcaCCCGGGUu -3' miRNA: 3'- aCGCgUCAuAUGGUG--UGUG--GGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 58793 | 0.66 | 0.889058 |
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Target: 5'- cGUGCAGgacUGCCGCaaACACCgCGGu- -3' miRNA: 3'- aCGCGUCau-AUGGUG--UGUGGgGCCcg -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 140131 | 0.66 | 0.889058 |
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Target: 5'- cGCGCcGUGUcggcggagGCagcggACGCGCUCCGGaGCg -3' miRNA: 3'- aCGCGuCAUA--------UGg----UGUGUGGGGCC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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