Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2713 | 5' | -56.8 | NC_001491.2 | + | 146484 | 0.72 | 0.555235 |
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Target: 5'- uUGCGuCGGggcccgaggGCCGCGCAgaCCCGGGUg -3' miRNA: 3'- -ACGC-GUCaua------UGGUGUGUg-GGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 8608 | 0.72 | 0.555235 |
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Target: 5'- cGCGCAcgaccagcggcGUAUGCCGUACAgCCCuGGCg -3' miRNA: 3'- aCGCGU-----------CAUAUGGUGUGUgGGGcCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 137380 | 0.72 | 0.535362 |
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Target: 5'- cUGCGaCAGUAagcGCCACGCGCCgCCgccugugccugcGGGCc -3' miRNA: 3'- -ACGC-GUCAUa--UGGUGUGUGG-GG------------CCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 52003 | 0.73 | 0.496422 |
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Target: 5'- aUGCGCGGU--ACCGCccaaaACCCCGuGGUa -3' miRNA: 3'- -ACGCGUCAuaUGGUGug---UGGGGC-CCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 65495 | 0.73 | 0.490689 |
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Target: 5'- --aGCAGUGUuggcGCCACAgGugccagccggggaagCCCCGGGCa -3' miRNA: 3'- acgCGUCAUA----UGGUGUgU---------------GGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 121659 | 0.76 | 0.340606 |
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Target: 5'- gGCGCAGaacgccCCugGC-CCCCGGGCc -3' miRNA: 3'- aCGCGUCauau--GGugUGuGGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 115726 | 0.78 | 0.276427 |
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Target: 5'- aGCGCAGggugGCCACcccgggccaccagGCuCCCCGGGCc -3' miRNA: 3'- aCGCGUCaua-UGGUG-------------UGuGGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 131279 | 0.98 | 0.012227 |
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Target: 5'- cUGCGCAGUAUACCACA-ACCCCGGGCu -3' miRNA: 3'- -ACGCGUCAUAUGGUGUgUGGGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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