Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2713 | 5' | -56.8 | NC_001491.2 | + | 23282 | 0.66 | 0.867028 |
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Target: 5'- gGCGC--UAUACCuucgcaaGCGCGCaaCGGGCg -3' miRNA: 3'- aCGCGucAUAUGG-------UGUGUGggGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 20227 | 0.68 | 0.774917 |
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Target: 5'- cUGgGCcuaaaUGUACCAUACACaaccuCCGGGCu -3' miRNA: 3'- -ACgCGuc---AUAUGGUGUGUGg----GGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 16282 | 0.7 | 0.656697 |
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Target: 5'- cGCGCgauAGcUGUACCAgGCGCCgagaGGGCg -3' miRNA: 3'- aCGCG---UC-AUAUGGUgUGUGGgg--CCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 13942 | 0.67 | 0.828172 |
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Target: 5'- aGcCGCuGGUAUAgCGCGCcggggcuggcgGCCuuGGGCg -3' miRNA: 3'- aC-GCG-UCAUAUgGUGUG-----------UGGggCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 12479 | 0.69 | 0.717109 |
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Target: 5'- gGCGCGGUAUGCCugGCAggcaacagccacUCCCacuGCa -3' miRNA: 3'- aCGCGUCAUAUGGugUGU------------GGGGcc-CG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 8608 | 0.72 | 0.555235 |
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Target: 5'- cGCGCAcgaccagcggcGUAUGCCGUACAgCCCuGGCg -3' miRNA: 3'- aCGCGU-----------CAUAUGGUGUGUgGGGcCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 2845 | 0.67 | 0.805776 |
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Target: 5'- aGCGCGccccGUACCcagugggcaauaaGCGCCUCGGGCg -3' miRNA: 3'- aCGCGUca--UAUGGug-----------UGUGGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 1914 | 0.71 | 0.585424 |
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Target: 5'- aGCGCgacgAGUAgacGCCGCGCGggUCCUGGGUg -3' miRNA: 3'- aCGCG----UCAUa--UGGUGUGU--GGGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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