Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2713 | 5' | -56.8 | NC_001491.2 | + | 116348 | 0.67 | 0.836475 |
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Target: 5'- gGCGCcgcuGGUGcGCUACaACGCcgagggggCCCGGGCc -3' miRNA: 3'- aCGCG----UCAUaUGGUG-UGUG--------GGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 34877 | 0.67 | 0.836475 |
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Target: 5'- gGCGCGGU--GCCAUgcuGCGCUUCaGGGUc -3' miRNA: 3'- aCGCGUCAuaUGGUG---UGUGGGG-CCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 146936 | 0.67 | 0.844593 |
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Target: 5'- gGCugGGUAUGCUgaACACGgCCUGGGUg -3' miRNA: 3'- aCGcgUCAUAUGG--UGUGUgGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 42551 | 0.66 | 0.85252 |
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Target: 5'- cUGcCGC-GUAUAUCucggcCGCAgCCUGGGCg -3' miRNA: 3'- -AC-GCGuCAUAUGGu----GUGUgGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 81240 | 0.66 | 0.85252 |
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Target: 5'- gGCGauuccCGGUGUugACCACGCGCCgCaGGUa -3' miRNA: 3'- aCGC-----GUCAUA--UGGUGUGUGGgGcCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 31794 | 0.66 | 0.85252 |
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Target: 5'- cGUGUAuGUAUGCCGCcuauauuCGCCUgGGGg -3' miRNA: 3'- aCGCGU-CAUAUGGUGu------GUGGGgCCCg -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 148964 | 0.66 | 0.855635 |
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Target: 5'- aGCGCGGcggagggccuggGCCACGCcggGgCUCGGGCc -3' miRNA: 3'- aCGCGUCaua---------UGGUGUG---UgGGGCCCG- -5' |
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| 2713 | 5' | -56.8 | NC_001491.2 | + | 140131 | 0.66 | 0.889058 |
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Target: 5'- cGCGCcGUGUcggcggagGCagcggACGCGCUCCGGaGCg -3' miRNA: 3'- aCGCGuCAUA--------UGg----UGUGUGGGGCC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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