Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27131 | 5' | -50.1 | NC_005832.1 | + | 95817 | 0.66 | 0.986524 |
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Target: 5'- aGGGGAggGCAcGUGUucaGGGcCCUCGAg -3' miRNA: 3'- -CCCCUa-UGUuUACGuagCUC-GGAGUU- -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 41471 | 0.66 | 0.98046 |
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Target: 5'- cGGG--GCAu-UGCGUCGAGgCUCAc -3' miRNA: 3'- cCCCuaUGUuuACGUAGCUCgGAGUu -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 10281 | 0.67 | 0.97256 |
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Target: 5'- gGGGGGUGaGGAUGaCcgUGuGCCUCAc -3' miRNA: 3'- -CCCCUAUgUUUAC-GuaGCuCGGAGUu -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 56614 | 0.67 | 0.969475 |
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Target: 5'- aGGGGAgaUGCcAagGCcUCGGGCCUgGAc -3' miRNA: 3'- -CCCCU--AUGuUuaCGuAGCUCGGAgUU- -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 50890 | 0.68 | 0.945691 |
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Target: 5'- aGGGGcgACAAGggaGCAaCGGGCgCUCGc -3' miRNA: 3'- -CCCCuaUGUUUa--CGUaGCUCG-GAGUu -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 74189 | 0.69 | 0.918433 |
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Target: 5'- cGGGAU-CGAGUGgAUCaGAGUCUCGu -3' miRNA: 3'- cCCCUAuGUUUACgUAG-CUCGGAGUu -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 66175 | 0.69 | 0.918433 |
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Target: 5'- cGGGGAcgu-AGUGCGUCcaGGGCUUCAGg -3' miRNA: 3'- -CCCCUauguUUACGUAG--CUCGGAGUU- -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 68596 | 0.69 | 0.918433 |
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Target: 5'- -cGGAUGCAGuGUGCGccgCGGGCCUUGAu -3' miRNA: 3'- ccCCUAUGUU-UACGUa--GCUCGGAGUU- -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 962 | 0.71 | 0.860589 |
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Target: 5'- uGGGGGaGCAGacGUGCGccUCGGGCUUCu- -3' miRNA: 3'- -CCCCUaUGUU--UACGU--AGCUCGGAGuu -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 82046 | 0.75 | 0.660291 |
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Target: 5'- aGGGGcacACGuAUGCGUCGcGCCUCAGa -3' miRNA: 3'- -CCCCua-UGUuUACGUAGCuCGGAGUU- -5' |
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| 27131 | 5' | -50.1 | NC_005832.1 | + | 100466 | 1.08 | 0.006672 |
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Target: 5'- gGGGGAUACAAAUGCAUCGAGCCUCAAc -3' miRNA: 3'- -CCCCUAUGUUUACGUAGCUCGGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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