Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27137 | 3' | -55 | NC_005832.1 | + | 6491 | 0.65 | 0.895959 |
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Target: 5'- aGGCgGcGGAUGACGaggacggGGUGUCUGuGGc -3' miRNA: 3'- -UUGgC-CCUGCUGCaa-----CCACAGACuCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 17136 | 0.66 | 0.889085 |
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Target: 5'- uGGCCGGGGCGGgGUUGa----UGAGAu -3' miRNA: 3'- -UUGGCCCUGCUgCAACcacagACUCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 62837 | 0.66 | 0.867038 |
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Target: 5'- gGGCUGGGACccuUGUUGGUGUCg---- -3' miRNA: 3'- -UUGGCCCUGcu-GCAACCACAGacucu -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 19061 | 0.67 | 0.851207 |
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Target: 5'- cAACUGGGACGACugcgaGUcGGUGUCUu--- -3' miRNA: 3'- -UUGGCCCUGCUG-----CAaCCACAGAcucu -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 5664 | 0.67 | 0.817078 |
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Target: 5'- -cCCGGGACc-CGUcGGUGguUCUGAGGa -3' miRNA: 3'- uuGGCCCUGcuGCAaCCAC--AGACUCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 91413 | 0.69 | 0.73078 |
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Target: 5'- cGCCGGGugGAUGgacaggaccaUGGccucccugugccUGUCUGAGGu -3' miRNA: 3'- uUGGCCCugCUGCa---------ACC------------ACAGACUCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 70942 | 0.72 | 0.553288 |
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Target: 5'- gAAgCGGGAUccgcgGACGUcagGGUGUUUGAGAg -3' miRNA: 3'- -UUgGCCCUG-----CUGCAa--CCACAGACUCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 87784 | 0.75 | 0.373296 |
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Target: 5'- gGACCuGGACGACGUUcacgugagucaGGUGUCUGuGGu -3' miRNA: 3'- -UUGGcCCUGCUGCAA-----------CCACAGACuCU- -5' |
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| 27137 | 3' | -55 | NC_005832.1 | + | 1281 | 1.06 | 0.003661 |
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Target: 5'- aAACCGGGACGACGUUGGUGUCUGAGAc -3' miRNA: 3'- -UUGGCCCUGCUGCAACCACAGACUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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