Results 61 - 67 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2717 | 3' | -63.9 | NC_001491.2 | + | 123135 | 0.66 | 0.559136 |
Target: 5'- gCGGCCAg-GCCUCCgcgggCGGCCcGGCGCc -3' miRNA: 3'- -GCCGGUggUGGGGGa----GCUGG-CCGUGc -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 8040 | 0.66 | 0.559136 |
Target: 5'- aCGGUUccuguGCCACCCCCUUaccucCUGGCAgGg -3' miRNA: 3'- -GCCGG-----UGGUGGGGGAGcu---GGCCGUgC- -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 101843 | 0.66 | 0.559136 |
Target: 5'- gCGGCUcaaGCgCGCCCCCggcgCGACCGucCACc -3' miRNA: 3'- -GCCGG---UG-GUGGGGGa---GCUGGCc-GUGc -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 147778 | 0.66 | 0.568604 |
Target: 5'- uGGCCGCCAgCUCCC-CGAagCGcGCGCc -3' miRNA: 3'- gCCGGUGGU-GGGGGaGCUg-GC-CGUGc -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 77626 | 0.66 | 0.568604 |
Target: 5'- uCGGCCGCUACCCCagUgGugCcucccacggaGGCACc -3' miRNA: 3'- -GCCGGUGGUGGGGg-AgCugG----------CCGUGc -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 38539 | 0.66 | 0.568604 |
Target: 5'- uCGGCCACCAgUCUUUCGGUCaGGUAUGu -3' miRNA: 3'- -GCCGGUGGUgGGGGAGCUGG-CCGUGC- -5' |
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2717 | 3' | -63.9 | NC_001491.2 | + | 9610 | 0.66 | 0.606815 |
Target: 5'- cCGGCCGCaccuaACCCCaCUgGGCUGGaucuCACc -3' miRNA: 3'- -GCCGGUGg----UGGGG-GAgCUGGCC----GUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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