Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27176 | 3' | -55.8 | NC_005832.1 | + | 79274 | 0.68 | 0.698824 |
Target: 5'- cACCCUCaggGAGACGua-GGCCUCAc -3' miRNA: 3'- aUGGGAGaa-CUCUGCccaUCGGAGUu -5' |
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27176 | 3' | -55.8 | NC_005832.1 | + | 97026 | 0.66 | 0.808698 |
Target: 5'- uUGCCCUC-UGuGGCGGGgagaaCCUCGc -3' miRNA: 3'- -AUGGGAGaACuCUGCCCauc--GGAGUu -5' |
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27176 | 3' | -55.8 | NC_005832.1 | + | 35009 | 1.04 | 0.003411 |
Target: 5'- cUACCCUCUUGAGACGGGUAGCCUCAAc -3' miRNA: 3'- -AUGGGAGAACUCUGCCCAUCGGAGUU- -5' |
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27176 | 3' | -55.8 | NC_005832.1 | + | 67239 | 0.66 | 0.789907 |
Target: 5'- gGCCCUgg-GAGAgGGGgcuGCCUCu- -3' miRNA: 3'- aUGGGAgaaCUCUgCCCau-CGGAGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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