Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27196 | 5' | -60.5 | NC_005832.1 | + | 810 | 0.66 | 0.666212 |
Target: 5'- aCAGGGgcugAGAGGugcagaagcccgaGGCgcaCGUCUgCUCCCc -3' miRNA: 3'- gGUCCC----UCUCC-------------CCGag-GCAGA-GAGGG- -5' |
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27196 | 5' | -60.5 | NC_005832.1 | + | 40017 | 0.66 | 0.677192 |
Target: 5'- cCCuuuGGGuAGAcGGGa-CCGUCgUCUCCCu -3' miRNA: 3'- -GGu--CCC-UCUcCCCgaGGCAG-AGAGGG- -5' |
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27196 | 5' | -60.5 | NC_005832.1 | + | 29992 | 0.65 | 0.683164 |
Target: 5'- cCCAGGGgcuacaGGAGGuucaagcagucuaGCUCUGUgUCUCCa -3' miRNA: 3'- -GGUCCC------UCUCCc------------CGAGGCAgAGAGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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