miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27199 3' -59.1 NC_005832.1 + 66692 0.67 0.569494
Target:  5'- aGCCCAgGCCGCggccGGGACCgUGUUuGGg- -3'
miRNA:   3'- aCGGGU-CGGUGa---CCUUGG-ACGA-CCaa -5'
27199 3' -59.1 NC_005832.1 + 48083 0.67 0.56638
Target:  5'- aGCCCgaggucaccgugguGGCC-CUGG-ACCUGgaGGUa -3'
miRNA:   3'- aCGGG--------------UCGGuGACCuUGGACgaCCAa -5'
27199 3' -59.1 NC_005832.1 + 100015 0.68 0.508262
Target:  5'- cGCUguuuaagaacggCAGgCACUGGAACgUGUUGGUg -3'
miRNA:   3'- aCGG------------GUCgGUGACCUUGgACGACCAa -5'
27199 3' -59.1 NC_005832.1 + 39864 0.68 0.508262
Target:  5'- cGUCUGGaCCcCUGGGACCUGCcGGa- -3'
miRNA:   3'- aCGGGUC-GGuGACCUUGGACGaCCaa -5'
27199 3' -59.1 NC_005832.1 + 11779 0.68 0.492389
Target:  5'- gGCUCGGCCGggGGGAggucgacgagggcacCCUGCUGGa- -3'
miRNA:   3'- aCGGGUCGGUgaCCUU---------------GGACGACCaa -5'
27199 3' -59.1 NC_005832.1 + 82261 0.69 0.449986
Target:  5'- gUGCCuuuaCAGCCggggcacuaacuGCUGGAGCacuaaCUGCUGGUg -3'
miRNA:   3'- -ACGG----GUCGG------------UGACCUUG-----GACGACCAa -5'
27199 3' -59.1 NC_005832.1 + 82386 0.7 0.40435
Target:  5'- gGUgCAGCUgguGCUGGAGCacuaaCUGCUGGUg -3'
miRNA:   3'- aCGgGUCGG---UGACCUUG-----GACGACCAa -5'
27199 3' -59.1 NC_005832.1 + 82461 0.7 0.40435
Target:  5'- gGUgCAGCUgguGCUGGAGCacuaaCUGCUGGUg -3'
miRNA:   3'- aCGgGUCGG---UGACCUUG-----GACGACCAa -5'
27199 3' -59.1 NC_005832.1 + 82341 0.7 0.40435
Target:  5'- gGUgCAGCUgguGCUGGAGCacuaaCUGCUGGUg -3'
miRNA:   3'- aCGgGUCGG---UGACCUUG-----GACGACCAa -5'
27199 3' -59.1 NC_005832.1 + 7513 0.7 0.40435
Target:  5'- cGUCCAGCCACccucccgccuugUGGAAggugucuagggUCUGCUGGg- -3'
miRNA:   3'- aCGGGUCGGUG------------ACCUU-----------GGACGACCaa -5'
27199 3' -59.1 NC_005832.1 + 62220 1.05 0.001384
Target:  5'- aUGCCCAGCCACUGGAACCUGCUGGUUc -3'
miRNA:   3'- -ACGGGUCGGUGACCUUGGACGACCAA- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.