Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 272 | 3' | -53.7 | AC_000008.1 | + | 16605 | 0.66 | 0.711341 |
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Target: 5'- --gCAGGAUUACAAGCcCCgaaagCUaaaGCGg -3' miRNA: 3'- ggaGUCCUAAUGUUCGaGGa----GGg--CGC- -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 22706 | 0.66 | 0.66625 |
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Target: 5'- gUUUAGGGUUGCcuccAGGCugcccgugUCCUCCUGCc -3' miRNA: 3'- gGAGUCCUAAUG----UUCG--------AGGAGGGCGc -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 32050 | 0.66 | 0.66625 |
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Target: 5'- gCUCAGGAaaGCaAAGUcagUCacaaUCCCGCGg -3' miRNA: 3'- gGAGUCCUaaUG-UUCG---AGg---AGGGCGC- -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 12935 | 0.67 | 0.643448 |
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Target: 5'- gCUguGGGgggUGCGGGCUCCcacaggcgaCCGCGc -3' miRNA: 3'- gGAguCCUa--AUGUUCGAGGag-------GGCGC- -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 33685 | 0.67 | 0.632025 |
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Target: 5'- cUCUCGGGuuccauggGCucuGCUCCUgCCGCc -3' miRNA: 3'- -GGAGUCCuaa-----UGuu-CGAGGAgGGCGc -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 24634 | 0.69 | 0.497778 |
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Target: 5'- gCUCAGGAgaggGC--GCUCCUagCCGCGc -3' miRNA: 3'- gGAGUCCUaa--UGuuCGAGGAg-GGCGC- -5' |
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| 272 | 3' | -53.7 | AC_000008.1 | + | 33762 | 1.1 | 0.00072 |
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Target: 5'- uCCUCAGGAUUACAAGCUCCUCCCGCGu -3' miRNA: 3'- -GGAGUCCUAAUGUUCGAGGAGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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