Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27200 | 5' | -57.6 | NC_005832.1 | + | 7916 | 0.66 | 0.782939 |
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Target: 5'- gAGGGUgaggacagacgacGUGUGGGAcGuGUACGagacccUGGGGc -3' miRNA: 3'- -UCCCA-------------CACACCCUuCcCAUGC------ACCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 28022 | 0.67 | 0.755532 |
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Target: 5'- cGGGaGUcGUGG--AGGGUA-GUGGGGg -3' miRNA: 3'- uCCCaCA-CACCcuUCCCAUgCACCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 1445 | 0.67 | 0.726209 |
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Target: 5'- gGGGGccUGUGgcugGGGAGGGG-ACGcUGGa- -3' miRNA: 3'- -UCCC--ACACa---CCCUUCCCaUGC-ACCcc -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 2664 | 0.67 | 0.716259 |
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Target: 5'- cGGGGUGgGUGuGGAAGGacacgaGgcCGUcGGGGg -3' miRNA: 3'- -UCCCACaCAC-CCUUCC------CauGCA-CCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 94 | 0.68 | 0.655357 |
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Target: 5'- uGGGaGUGggGGGAucugguuuGGGGggaGUGGGGg -3' miRNA: 3'- uCCCaCACa-CCCU--------UCCCaugCACCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 6303 | 0.69 | 0.634813 |
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Target: 5'- gAGGGUaGUGUGccucgagaacGGAGGGG-ACGcggcGGGGa -3' miRNA: 3'- -UCCCA-CACAC----------CCUUCCCaUGCa---CCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 79966 | 0.69 | 0.604 |
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Target: 5'- uGGGacagGUGGaGGAGGG-AgGUGGGGg -3' miRNA: 3'- uCCCaca-CACC-CUUCCCaUgCACCCC- -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 62030 | 0.69 | 0.593761 |
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Target: 5'- gAGGGUGUGgGGGAagcagaacaucaGGGcGUugGUGaGGu -3' miRNA: 3'- -UCCCACACaCCCU------------UCC-CAugCAC-CCc -5' |
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| 27200 | 5' | -57.6 | NC_005832.1 | + | 64975 | 1.09 | 0.001416 |
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Target: 5'- gAGGGUGUGUGGGAAGGGUACGUGGGGc -3' miRNA: 3'- -UCCCACACACCCUUCCCAUGCACCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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