Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27203 | 3' | -56.2 | NC_005832.1 | + | 66339 | 0.66 | 0.8237 |
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Target: 5'- --cAGCCGUCUUCGCCcAUGcccguccugcccaGGUGCAg -3' miRNA: 3'- guaUCGGCAGGGGUGGaUGC-------------UCACGU- -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 65524 | 0.66 | 0.815756 |
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Target: 5'- ---uGCCGUCCCUGCgaAgGAGUGgGu -3' miRNA: 3'- guauCGGCAGGGGUGgaUgCUCACgU- -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 47283 | 0.66 | 0.797603 |
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Target: 5'- aCGUGGUgacUGaCCCCGCCgacggaaaGCGAGUGCc -3' miRNA: 3'- -GUAUCG---GCaGGGGUGGa-------UGCUCACGu -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 48275 | 0.67 | 0.759479 |
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Target: 5'- --aGGCCGgCUUCGCCUGCcaccAGUGCAg -3' miRNA: 3'- guaUCGGCaGGGGUGGAUGc---UCACGU- -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 1034 | 0.69 | 0.678163 |
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Target: 5'- --cGGCCGgCCCCugCUACGccgggGGcUGCAg -3' miRNA: 3'- guaUCGGCaGGGGugGAUGC-----UC-ACGU- -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 81244 | 0.69 | 0.667717 |
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Target: 5'- uUAUGGCCaugaaccaCCCCACCUGgGAGUcuGCGg -3' miRNA: 3'- -GUAUCGGca------GGGGUGGAUgCUCA--CGU- -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 94349 | 0.7 | 0.604715 |
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Target: 5'- uGUGGUgaucaCGUCuCCCACCuUGCGGGUGUc -3' miRNA: 3'- gUAUCG-----GCAG-GGGUGG-AUGCUCACGu -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 54833 | 0.75 | 0.348862 |
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Target: 5'- ----cCCGUCCCCACCUugGAGUa-- -3' miRNA: 3'- guaucGGCAGGGGUGGAugCUCAcgu -5' |
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| 27203 | 3' | -56.2 | NC_005832.1 | + | 65531 | 1.08 | 0.001878 |
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Target: 5'- aCAUAGCCGUCCCCACCUACGAGUGCAu -3' miRNA: 3'- -GUAUCGGCAGGGGUGGAUGCUCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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