Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27209 | 5' | -55.1 | NC_005832.1 | + | 92745 | 0.67 | 0.844437 |
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Target: 5'- aGGGACAgcCUCCUGGUGagGAGUUu-- -3' miRNA: 3'- -CCCUGUa-GAGGAUCGCagCUCGGucu -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 44787 | 0.67 | 0.818777 |
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Target: 5'- uGGGGagcCUCCUGccCGUCGAGCCcGAg -3' miRNA: 3'- -CCCUguaGAGGAUc-GCAGCUCGGuCU- -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 95767 | 0.68 | 0.78211 |
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Target: 5'- aGGAgCGUCUCCUAGgagaaUGUCuccuGCCAGAg -3' miRNA: 3'- cCCU-GUAGAGGAUC-----GCAGcu--CGGUCU- -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 77305 | 0.69 | 0.712873 |
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Target: 5'- aGGGCAcCUC--GGCGUCGGGCCGa- -3' miRNA: 3'- cCCUGUaGAGgaUCGCAGCUCGGUcu -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 48175 | 0.7 | 0.702613 |
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Target: 5'- aGGACGUCUgaggCCUuucgGGCGUCGAaCCAGc -3' miRNA: 3'- cCCUGUAGA----GGA----UCGCAGCUcGGUCu -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 86129 | 0.7 | 0.681916 |
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Target: 5'- aGGaGACAaagauuUCUCCUAGCa--GAGUCAGAc -3' miRNA: 3'- -CC-CUGU------AGAGGAUCGcagCUCGGUCU- -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 15374 | 0.7 | 0.659999 |
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Target: 5'- uGGGCugcUCUCCccUGGCGUCGAGCaccuuuguguacuCAGAu -3' miRNA: 3'- cCCUGu--AGAGG--AUCGCAGCUCG-------------GUCU- -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 53867 | 0.71 | 0.608597 |
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Target: 5'- cGGGcAUGUCUCCaucuaucGCGUCGAGCCu-- -3' miRNA: 3'- -CCC-UGUAGAGGau-----CGCAGCUCGGucu -5' |
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| 27209 | 5' | -55.1 | NC_005832.1 | + | 76025 | 1.12 | 0.001614 |
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Target: 5'- cGGGACAUCUCCUAGCGUCGAGCCAGAg -3' miRNA: 3'- -CCCUGUAGAGGAUCGCAGCUCGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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