Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27210 | 3' | -58.2 | NC_005832.1 | + | 56147 | 0.67 | 0.653444 |
Target: 5'- -cGUCUCCUGG-AGCUUgacggCCguGGUa -3' miRNA: 3'- gaCAGAGGGCCaUCGAGa----GGguCCAa -5' |
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27210 | 3' | -58.2 | NC_005832.1 | + | 41978 | 0.68 | 0.601256 |
Target: 5'- cCUGgccaUCgCCGGggugAGCuUCUCCCAGGg- -3' miRNA: 3'- -GACag--AG-GGCCa---UCG-AGAGGGUCCaa -5' |
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27210 | 3' | -58.2 | NC_005832.1 | + | 26333 | 0.68 | 0.55988 |
Target: 5'- --cUCUgCCCGGUGGC-CUCgCCGGGg- -3' miRNA: 3'- gacAGA-GGGCCAUCGaGAG-GGUCCaa -5' |
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27210 | 3' | -58.2 | NC_005832.1 | + | 94252 | 0.69 | 0.52937 |
Target: 5'- -cGUCUCCCcu--GCUCUCCCAGa-- -3' miRNA: 3'- gaCAGAGGGccauCGAGAGGGUCcaa -5' |
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27210 | 3' | -58.2 | NC_005832.1 | + | 10462 | 0.69 | 0.499493 |
Target: 5'- -aGUCUCCCugccGGUGGCUCUCCUc---- -3' miRNA: 3'- gaCAGAGGG----CCAUCGAGAGGGuccaa -5' |
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27210 | 3' | -58.2 | NC_005832.1 | + | 78104 | 1.05 | 0.002049 |
Target: 5'- cCUGUCUCCCGGUAGCUCUCCCAGGUUc -3' miRNA: 3'- -GACAGAGGGCCAUCGAGAGGGUCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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