Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27216 | 5' | -60.6 | NC_005832.1 | + | 39434 | 0.66 | 0.613476 |
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Target: 5'- uCCGGucGGG-UCCGUG-GGAGCGGCu- -3' miRNA: 3'- uGGUC--CCCuGGGCACuCCUUGCCGua -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 19614 | 0.66 | 0.613476 |
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Target: 5'- cGCCgcaggAGGGaGACCCGUugaagguggGAGacGAGCGGCGg -3' miRNA: 3'- -UGG-----UCCC-CUGGGCA---------CUC--CUUGCCGUa -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 40902 | 0.66 | 0.582878 |
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Target: 5'- uGCCaaaggAGGGGACCCGaccGGGuaAGCGGCc- -3' miRNA: 3'- -UGG-----UCCCCUGGGCac-UCC--UUGCCGua -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 39542 | 0.66 | 0.579833 |
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Target: 5'- uCCGGGaGGACCCGcggGAccagugggagacauGGAAcCGGCAg -3' miRNA: 3'- uGGUCC-CCUGGGCa--CU--------------CCUU-GCCGUa -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 1454 | 0.66 | 0.57274 |
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Target: 5'- gACCaagaGGGGGGCCUGUGgcuGGGGAgGGgAc -3' miRNA: 3'- -UGG----UCCCCUGGGCAC---UCCUUgCCgUa -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 29319 | 0.66 | 0.562645 |
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Target: 5'- gACCgaggagAGGGGACCUcUGAGGuGCuGGCGc -3' miRNA: 3'- -UGG------UCCCCUGGGcACUCCuUG-CCGUa -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 39588 | 0.68 | 0.474642 |
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Target: 5'- cCCAGGGGACCUGc-AGGAGaGGCc- -3' miRNA: 3'- uGGUCCCCUGGGCacUCCUUgCCGua -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 90555 | 0.68 | 0.446828 |
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Target: 5'- --gAGGGGugCC-UGAGGGACGGgAg -3' miRNA: 3'- uggUCCCCugGGcACUCCUUGCCgUa -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 1665 | 0.69 | 0.394065 |
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Target: 5'- cGCCGaGGGuguagucguaguGCUCGUGAGGAcCGGCGUa -3' miRNA: 3'- -UGGUcCCC------------UGGGCACUCCUuGCCGUA- -5' |
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| 27216 | 5' | -60.6 | NC_005832.1 | + | 80199 | 1.05 | 0.001156 |
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Target: 5'- gACCAGGGGACCCGUGAGGAACGGCAUa -3' miRNA: 3'- -UGGUCCCCUGGGCACUCCUUGCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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