Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27219 | 5' | -59.3 | NC_005832.1 | + | 103736 | 0.67 | 0.571701 |
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Target: 5'- -cGcCAGCUGGUggaGCUGaGUGCAGCccaugagaucggccaUGGa -3' miRNA: 3'- caC-GUCGACCA---CGAC-CACGUCG---------------ACCa -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 17886 | 0.67 | 0.557186 |
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Target: 5'- uUGCuGCUGcUGCUGcUGCuGCUGGa -3' miRNA: 3'- cACGuCGACcACGACcACGuCGACCa -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 82472 | 0.77 | 0.132351 |
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Target: 5'- ----uGCUGGagcacuaacUGCUGGUGCAGCUGGUg -3' miRNA: 3'- cacguCGACC---------ACGACCACGUCGACCA- -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 82326 | 1.07 | 0.000892 |
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Target: 5'- gGUGCAGCUGGUGCUGGUGCAGCUGGUg -3' miRNA: 3'- -CACGUCGACCACGACCACGUCGACCA- -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 82371 | 1.07 | 0.000892 |
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Target: 5'- gGUGCAGCUGGUGCUGGUGCAGCUGGUg -3' miRNA: 3'- -CACGUCGACCACGACCACGUCGACCA- -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 82416 | 1.07 | 0.000892 |
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Target: 5'- gGUGCAGCUGGUGCUGGUGCAGCUGGUg -3' miRNA: 3'- -CACGUCGACCACGACCACGUCGACCA- -5' |
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| 27219 | 5' | -59.3 | NC_005832.1 | + | 82446 | 1.07 | 0.000892 |
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Target: 5'- gGUGCAGCUGGUGCUGGUGCAGCUGGUg -3' miRNA: 3'- -CACGUCGACCACGACCACGUCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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