Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27220 | 3' | -57 | NC_005832.1 | + | 67027 | 0.68 | 0.640078 |
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Target: 5'- gGCCCUggcggcGGccGUGGCGaGGGCCGcGGGc -3' miRNA: 3'- -UGGGAa-----CUucCACCGC-CCCGGUuUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 68034 | 0.69 | 0.608597 |
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Target: 5'- gGCCUUUGccuccGAGGUGGaggagaGGGGCgGAGGc -3' miRNA: 3'- -UGGGAAC-----UUCCACCg-----CCCCGgUUUCu -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 79157 | 0.69 | 0.59813 |
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Target: 5'- uCCC-UGAGGGUGa--GGGCCAGGGAc -3' miRNA: 3'- uGGGaACUUCCACcgcCCCGGUUUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 80817 | 0.68 | 0.681916 |
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Target: 5'- gGCCC-UGGAGG-GGaCGGGGuCCucguAGAc -3' miRNA: 3'- -UGGGaACUUCCaCC-GCCCC-GGuu--UCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 80969 | 0.73 | 0.376791 |
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Target: 5'- cGCCC-UGAGGGUGGCGucGGUCAAcgGGAc -3' miRNA: 3'- -UGGGaACUUCCACCGCc-CCGGUU--UCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 83265 | 1.08 | 0.001525 |
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Target: 5'- uACCCUUGAAGGUGGCGGGGCCAAAGAg -3' miRNA: 3'- -UGGGAACUUCCACCGCCCCGGUUUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 95321 | 0.68 | 0.681916 |
|
Target: 5'- cGCCaCggacaaggGAGGGUGGCuGGGGUCAGc-- -3' miRNA: 3'- -UGG-Gaa------CUUCCACCG-CCCCGGUUucu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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