Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27220 | 3' | -57 | NC_005832.1 | + | 79157 | 0.69 | 0.59813 |
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Target: 5'- uCCC-UGAGGGUGa--GGGCCAGGGAc -3' miRNA: 3'- uGGGaACUUCCACcgcCCCGGUUUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 12106 | 0.69 | 0.587689 |
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Target: 5'- gACCCcaugUUGGAGGgGGCGGGGU---AGAc -3' miRNA: 3'- -UGGG----AACUUCCaCCGCCCCGguuUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 80969 | 0.73 | 0.376791 |
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Target: 5'- cGCCC-UGAGGGUGGCGucGGUCAAcgGGAc -3' miRNA: 3'- -UGGGaACUUCCACCGCc-CCGGUU--UCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 7952 | 0.74 | 0.328642 |
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Target: 5'- gACCC-UGggGcUGGCGGcGGCCAaaAAGAg -3' miRNA: 3'- -UGGGaACuuCcACCGCC-CCGGU--UUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 26608 | 0.75 | 0.299154 |
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Target: 5'- gGCCUUU-AAGGUGGCGaGGGCCGccGGg -3' miRNA: 3'- -UGGGAAcUUCCACCGC-CCCGGUuuCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 28059 | 0.76 | 0.25883 |
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Target: 5'- gGCCCUguccAGGGUGGUGGGGaCCA-AGAg -3' miRNA: 3'- -UGGGAac--UUCCACCGCCCC-GGUuUCU- -5' |
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| 27220 | 3' | -57 | NC_005832.1 | + | 83265 | 1.08 | 0.001525 |
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Target: 5'- uACCCUUGAAGGUGGCGGGGCCAAAGAg -3' miRNA: 3'- -UGGGAACUUCCACCGCCCCGGUUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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