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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27220 | 5' | -58.5 | NC_005832.1 | + | 54882 | 0.66 | 0.723825 |
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Target: 5'- -gUCgacGUCCgCCgccaggcacagGCCGGUCCUCUCg -3' miRNA: 3'- gaAGauaCAGG-GG-----------CGGCCGGGAGAGa -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 39217 | 0.66 | 0.723825 |
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Target: 5'- --cCUGggGUCCCgGaCGGCCCUC-CUg -3' miRNA: 3'- gaaGAUa-CAGGGgCgGCCGGGAGaGA- -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 43490 | 0.67 | 0.673123 |
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Target: 5'- ------cGUCCCCGUCGGaCUUUCUCc -3' miRNA: 3'- gaagauaCAGGGGCGGCC-GGGAGAGa -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 71001 | 0.67 | 0.662844 |
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Target: 5'- ------gGUCCCCGUUaGGCCCcCUCUg -3' miRNA: 3'- gaagauaCAGGGGCGG-CCGGGaGAGA- -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 31343 | 0.68 | 0.611241 |
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Target: 5'- -gUCUGUGUUCUCGa-GGCCCUUUUUu -3' miRNA: 3'- gaAGAUACAGGGGCggCCGGGAGAGA- -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 50501 | 0.68 | 0.611241 |
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Target: 5'- gUUCag-GUCCCCG-CGGCCCUUa-- -3' miRNA: 3'- gAAGauaCAGGGGCgGCCGGGAGaga -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 19352 | 0.7 | 0.471484 |
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Target: 5'- aUUCUcgGUcagguauccucCCCUGauGGCCCUCUCUg -3' miRNA: 3'- gAAGAuaCA-----------GGGGCggCCGGGAGAGA- -5' |
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| 27220 | 5' | -58.5 | NC_005832.1 | + | 83303 | 1.07 | 0.001609 |
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Target: 5'- uCUUCUAUGUCCCCGCCGGCCCUCUCUc -3' miRNA: 3'- -GAAGAUACAGGGGCGGCCGGGAGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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