Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27222 | 5' | -47.8 | NC_005832.1 | + | 25657 | 0.66 | 0.997912 |
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Target: 5'- aGGuCGGGgggACAUgg-GGUUCGGGguACc -3' miRNA: 3'- -UC-GUCCa--UGUGaaaCCAAGUCCuuUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 80110 | 0.66 | 0.996989 |
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Target: 5'- aGGCucAGGUACA----GGUcCAGGAGACc -3' miRNA: 3'- -UCG--UCCAUGUgaaaCCAaGUCCUUUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 102055 | 0.66 | 0.996413 |
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Target: 5'- gAGCAGcgGCA---UGGcUCAGGAGACg -3' miRNA: 3'- -UCGUCcaUGUgaaACCaAGUCCUUUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 104137 | 0.66 | 0.99575 |
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Target: 5'- uGCAGGUACACcg----UCAGGAc-- -3' miRNA: 3'- uCGUCCAUGUGaaaccaAGUCCUuug -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 63756 | 0.67 | 0.994991 |
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Target: 5'- -aCAGGgACAUcagaaagaGGUUCAGGAAACu -3' miRNA: 3'- ucGUCCaUGUGaaa-----CCAAGUCCUUUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 21275 | 0.68 | 0.984189 |
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Target: 5'- cGCgGGGUACAUaggGGUgaUCAGGAcaGACa -3' miRNA: 3'- uCG-UCCAUGUGaaaCCA--AGUCCU--UUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 35257 | 0.69 | 0.981864 |
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Target: 5'- aGGCAGGgACAggcUGGUUCucuuugcGGGAGACg -3' miRNA: 3'- -UCGUCCaUGUgaaACCAAG-------UCCUUUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 90181 | 0.69 | 0.979778 |
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Target: 5'- aAGguGGUGCuCUUUuggacgGGgaCGGGAAACa -3' miRNA: 3'- -UCguCCAUGuGAAA------CCaaGUCCUUUG- -5' |
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| 27222 | 5' | -47.8 | NC_005832.1 | + | 86842 | 1.11 | 0.008065 |
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Target: 5'- gAGCAGGUACACUUUGGUUCAGGAAACu -3' miRNA: 3'- -UCGUCCAUGUGAAACCAAGUCCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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