Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27226 | 5' | -54.5 | NC_005832.1 | + | 92136 | 0.66 | 0.869702 |
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Target: 5'- -gGuGGGAGAGUUUuggagggcggugGCAGACaggauagaGUCCGGg -3' miRNA: 3'- caC-CCCUCUCAAA------------CGUCUG--------CAGGUCa -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 39449 | 0.66 | 0.869702 |
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Target: 5'- -cGGGGGGGGUcaUGCuAGACccgCCAGg -3' miRNA: 3'- caCCCCUCUCAa-ACG-UCUGca-GGUCa -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 75091 | 0.66 | 0.859493 |
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Target: 5'- -gGGGGaAGAGUaaccgaaccagcccUUGCAGGCGUagucucUCAGUu -3' miRNA: 3'- caCCCC-UCUCA--------------AACGUCUGCA------GGUCA- -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 10787 | 0.67 | 0.819555 |
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Target: 5'- gGUGGGGAucGgcUGCGGAgG-CCAGg -3' miRNA: 3'- -CACCCCUcuCaaACGUCUgCaGGUCa -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 10312 | 0.7 | 0.690969 |
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Target: 5'- -aGGGGAGAGgcggugGCAGucacCGUCCAc- -3' miRNA: 3'- caCCCCUCUCaaa---CGUCu---GCAGGUca -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 21461 | 0.7 | 0.680415 |
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Target: 5'- -cGGGGGGAGUcuacccUGCuguauGGCGUCCAuGUa -3' miRNA: 3'- caCCCCUCUCAa-----ACGu----CUGCAGGU-CA- -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 45870 | 0.73 | 0.522031 |
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Target: 5'- -aGGGGAGAcGgugGCAGACGgcaCCAGg -3' miRNA: 3'- caCCCCUCU-CaaaCGUCUGCa--GGUCa -5' |
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| 27226 | 5' | -54.5 | NC_005832.1 | + | 89350 | 1.07 | 0.003245 |
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Target: 5'- cGUGGGGAGAGUUUGCAGACGUCCAGUc -3' miRNA: 3'- -CACCCCUCUCAAACGUCUGCAGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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