Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27228 | 5' | -55.7 | NC_005832.1 | + | 74642 | 0.66 | 0.842291 |
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Target: 5'- gCGGGcaGgaGCAgGAGGAggugacuGCUCUCCGCu -3' miRNA: 3'- -GCCC--CaaCGUgCUCUU-------CGAGAGGUGc -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 6701 | 0.67 | 0.826201 |
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Target: 5'- aCGGGG--GCAgGAGGAGCcUCccCCACu -3' miRNA: 3'- -GCCCCaaCGUgCUCUUCG-AGa-GGUGc -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 2665 | 0.67 | 0.817461 |
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Target: 5'- aCGGGGUggguguggaagGaCACGAGGccgucgggGGCUC-CCACa -3' miRNA: 3'- -GCCCCAa----------C-GUGCUCU--------UCGAGaGGUGc -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 6838 | 0.67 | 0.799463 |
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Target: 5'- uGGGGUaGUggGGGAGGCUcCUCCuGCc -3' miRNA: 3'- gCCCCAaCGugCUCUUCGA-GAGG-UGc -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 56126 | 0.68 | 0.780833 |
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Target: 5'- gGcGGGUgcaaaGUACGAG-AGCgUCUCCugGa -3' miRNA: 3'- gC-CCCAa----CGUGCUCuUCG-AGAGGugC- -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 7410 | 0.68 | 0.780833 |
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Target: 5'- gCGGGGUccgUGCccuuCGGGGAGC--UCCugGg -3' miRNA: 3'- -GCCCCA---ACGu---GCUCUUCGagAGGugC- -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 49363 | 0.68 | 0.741978 |
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Target: 5'- uGGGGcUGU--GAGAAGaCUCUCgCACGg -3' miRNA: 3'- gCCCCaACGugCUCUUC-GAGAG-GUGC- -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 43289 | 0.73 | 0.47625 |
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Target: 5'- uGGGGUUcGUGCGGGAGGCcaggcucugUCUCCuCGg -3' miRNA: 3'- gCCCCAA-CGUGCUCUUCG---------AGAGGuGC- -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 71160 | 0.75 | 0.360368 |
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Target: 5'- aGGG---GCugGAGAGGCUCUCCGgCGg -3' miRNA: 3'- gCCCcaaCGugCUCUUCGAGAGGU-GC- -5' |
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| 27228 | 5' | -55.7 | NC_005832.1 | + | 90197 | 1.08 | 0.002383 |
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Target: 5'- uCGGGGUUGCACGAGAAGCUCUCCACGu -3' miRNA: 3'- -GCCCCAACGUGCUCUUCGAGAGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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