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miRNA: 3'- gACGGAUG-ACCGGUUuuu-CUugCUGG- -5'
miRNA: 3'- gaCGG-------AUGACCGGUUUUuCUUGCUGG- -5'
miRNA: 3'- gACGGa--UG----ACCGGUUUUUCUUGCUGg -5'
miRNA: 3'- -GACGGa----UGaCCGGUUUUUCU--UGCUGG- -5'
miRNA: 3'- -GACGGAUGACCGGUUUUUCUUGCUGG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 27233 | 5' | -49 | NC_005832.1 | + | 71269 | 0.76 | 0.7137 |
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Target: 5'- gUGUCUGCgUGGCCAAugucGGugGACCc -3' miRNA: 3'- gACGGAUG-ACCGGUUuuu-CUugCUGG- -5' |
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| 27233 | 5' | -49 | NC_005832.1 | + | 96766 | 0.77 | 0.636153 |
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Target: 5'- -cGUCagggagaUGCUGGCCAAGGuGGACGGCCu -3' miRNA: 3'- gaCGG-------AUGACCGGUUUUuCUUGCUGG- -5' |
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| 27233 | 5' | -49 | NC_005832.1 | + | 50353 | 0.8 | 0.496859 |
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Target: 5'- gUGCUgggACgaagUGGCCAAAAAGGACGACa -3' miRNA: 3'- gACGGa--UG----ACCGGUUUUUCUUGCUGg -5' |
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| 27233 | 5' | -49 | NC_005832.1 | + | 79867 | 0.84 | 0.306564 |
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Target: 5'- aCUGCCgaggaGCaGGCCGAGGAGAggGCGGCCa -3' miRNA: 3'- -GACGGa----UGaCCGGUUUUUCU--UGCUGG- -5' |
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| 27233 | 5' | -49 | NC_005832.1 | + | 97232 | 1.13 | 0.004881 |
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Target: 5'- cCUGCCUACUGGCCAAAAAGAACGACCu -3' miRNA: 3'- -GACGGAUGACCGGUUUUUCUUGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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